Alternative titles; symbols
HGNC Approved Gene Symbol: H4C14
Cytogenetic location: 1q21.2 Genomic coordinates (GRCh38): 1:149,832,657-149,833,052 (from NCBI)
For background information on histones, histone gene clusters, and the H4 histone family, see HIST1H4A (602822).
By restriction endonuclease digestion of a genomic clone, Sierra et al. (1983) isolated DNA (pF0108) with the capacity to code for a typical H4 histone protein, H4FN. Sequence analysis predicted a 103-amino acid protein. Autoradiographic analysis of an in vitro transcribed pF0108 allowed visualization of a 2.9-kb transcript.
By genomic sequence analysis, Marzluff et al. (2002) identified the mouse and human HIST2H4A genes. All mouse and human H4 genes, including HIST2H4A, encode the same protein.
Sierra et al. (1983) found that H4FN has a 5-prime promoter containing regulatory sequences typically used by RNA polymerase II. The H4FN gene contains no intervening sequences. Sierra et al. (1983) predicted the termination of the mRNA at the ACCA motif just downstream from the hyphenated dyad symmetry characteristic of other histone genes.
Pauli et al. (1987) identified 2 regions of DNA with 4 potential protein-binding domains in the 5-prime promoter region of H4FN that are protected from reaction with dimethyl sulfate in cells and from digestion with DNase I in nuclei.
By genomic sequence analysis, Marzluff et al. (2002) determined that the histone gene cluster on chromosome 1q21, which they called histone gene cluster-2 (HIST2), contains 6 histone genes, including HIST2H4A.
See HIST1H4A (602822) for functional information on H4 histones.
Marzluff, W. F., Gongidi, P., Woods, K. R., Jin, J., Maltais, L. J. The human and mouse replication-dependent histone genes. Genomics 80: 487-498, 2002. [PubMed: 12408966]
Pauli, U., Chrysogelos, S., Stein, G., Stein, J., Nick, H. Protein-DNA interactions in vivo upstream of a cell cycle-regulated human H4 histone gene. Science 236: 1308-1311, 1987. [PubMed: 3035717] [Full Text: https://doi.org/10.1126/science.3035717]
Sierra, F., Stein, G., Stein, J. Structure and in vitro transcription of a human H4 histone gene. Nucleic Acids Res. 11: 7069-7086, 1983. [PubMed: 6314274] [Full Text: https://doi.org/10.1093/nar/11.20.7069]