Alternative titles; symbols
HGNC Approved Gene Symbol: NEFM
Cytogenetic location: 8p21.2 Genomic coordinates (GRCh38): 8:24,913,761-24,919,093 (from NCBI)
NEF3 is an intermediate-sized cytoskeletal neurofilament that localizes to neuronal axons and dendrites (Myers et al., 1987).
By sequencing clones obtained from a 1-day-old infant basal ganglia cDNA library and a genomic library, Myers et al. (1987) cloned NEF3, which they called NFM. The deduced 915-amino acid protein has a calculated molecular mass of approximately 102 kD. NEF3 has an N-terminal alpha-helical region and a C-terminal repeat domain. It had an apparent molecular mass of 150 kD by SDS-PAGE.
Myers et al. (1987) determined that the NEF3 gene contains 3 exons. They identified a TATAAA motif at the proximal 5-prime UTR.
Hartz (2014) mapped the NEF3 gene to chromosome 8p21.2 based on an alignment of the NEF3 sequence (GenBank BC002421) with the genomic sequence (GRCh37).
Levy et al. (1987) found that the mouse Nef3 and Nefl (162280) genes appeared to be on chromosome 14.
Hartz, P. A. Personal Communication. Baltimore, Md. 5/21/2014.
Levy, E., Liem, R. K. H., D'Eustachio, P., Cowan, N. J. Structure and evolutionary origin of the gene encoding NF-M, the middle-molecular-mass neurofilament protein. Europ. J. Biochem. 166: 71-77, 1987. [PubMed: 3036526] [Full Text: https://doi.org/10.1111/j.1432-1033.1987.tb13485.x]
Myers, M. W., Lazzarini, R. A., Lee, V. M.-Y., Schlaepfer, W. W., Nelson, D. L. The human mid-size neurofilament subunit: a repeated protein sequence and the relationship of its gene to the intermediate filament gene family. EMBO J. 6: 1617-1626, 1987. [PubMed: 3608989] [Full Text: https://doi.org/10.1002/j.1460-2075.1987.tb02409.x]