Entry - *180661 - POLYMERASE II, RNA, SUBUNIT B; POLR2B - OMIM

 
 
* 180661

POLYMERASE II, RNA, SUBUNIT B; POLR2B


Alternative titles; symbols

RPB2, S. CEREVISIAE, HOMOLOG OF
RNA POLYMERASE II, 140-KD SUBUNIT
RNA POLYMERASE II, SECOND LARGEST SUBUNIT


HGNC Approved Gene Symbol: POLR2B

Cytogenetic location: 4q12     Genomic coordinates (GRCh38): 4:56,978,896-57,031,158 (from NCBI)


▼ TEXT

The DNA-dependent RNA polymerase II (EC 2.7.7.6) is responsible for the transcription of protein-encoding genes. It is composed of 10 to 14 subunits ranging in mass from approximately 220 kD (POLR2A; 180660) to approximately 7 kD (POLR2K; 606033). For general information on the structure and function of RNA polymerase II, see 180660.

▼ Cloning and Expression

Acker et al. (1992) cloned and sequenced the cDNA for the second largest subunit of RNA polymerase II (or B) from HeLa cells. The predicted amino acid sequence of 1,174 residues (calculated molecular mass of 133,896 Da) was derived from the longest open reading frame and compared to the sequences of homologous subunits of polymerases of eukaryotic, archaic, and bacterial origin.


▼ Mapping

By in situ hybridization, Acker et al. (1994) assigned the POLR2B gene to chromosome 4q12.


▼ See Also:

▼ REFERENCES

  1. Acker, J., Mattei, M.-G., Wintzerith, M., Roeckel, N., Depetris, D., Vigneron, M., Kedinger, C. Chromosomal localization of human RNA polymerase II subunit genes. Genomics 20: 496-499, 1994. [PubMed: 8034326, related citations] [Full Text]

  2. Acker, J., Wintzerith, M., Vigneron, M., Kedinger, C. Primary structure of the second largest subunit of human RNA polymerase II (or B). J. Molec. Biol. 226: 1295-1299, 1992. [PubMed: 1518060, related citations] [Full Text]

  3. Hsin, J.-P., Sheth, A., Manley, J. L. RNAP II CTD phosphorylated on threonine-4 is required for histone mRNA 3-prime end processing. Science 334: 683-686, 2011. [PubMed: 22053051, images, related citations] [Full Text]


Creation Date:
Victor A. McKusick : 6/23/1993
Edit History:
alopez : 11/30/2011
terry : 11/29/2011
carol : 6/19/2001
alopez : 6/19/2001
carol : 6/19/2001
alopez : 6/19/2001
alopez : 6/19/2001
carol : 6/15/2001
psherman : 9/2/1999
carol : 6/22/1998
joanna : 4/4/1996
carol : 4/18/1994
carol : 6/23/1993

* 180661

POLYMERASE II, RNA, SUBUNIT B; POLR2B


Alternative titles; symbols

RPB2, S. CEREVISIAE, HOMOLOG OF
RNA POLYMERASE II, 140-KD SUBUNIT
RNA POLYMERASE II, SECOND LARGEST SUBUNIT


HGNC Approved Gene Symbol: POLR2B

Cytogenetic location: 4q12     Genomic coordinates (GRCh38): 4:56,978,896-57,031,158 (from NCBI)


TEXT

The DNA-dependent RNA polymerase II (EC 2.7.7.6) is responsible for the transcription of protein-encoding genes. It is composed of 10 to 14 subunits ranging in mass from approximately 220 kD (POLR2A; 180660) to approximately 7 kD (POLR2K; 606033). For general information on the structure and function of RNA polymerase II, see 180660.

Cloning and Expression

Acker et al. (1992) cloned and sequenced the cDNA for the second largest subunit of RNA polymerase II (or B) from HeLa cells. The predicted amino acid sequence of 1,174 residues (calculated molecular mass of 133,896 Da) was derived from the longest open reading frame and compared to the sequences of homologous subunits of polymerases of eukaryotic, archaic, and bacterial origin.


Mapping

By in situ hybridization, Acker et al. (1994) assigned the POLR2B gene to chromosome 4q12.


See Also:

Hsin et al. (2011)

REFERENCES

  1. Acker, J., Mattei, M.-G., Wintzerith, M., Roeckel, N., Depetris, D., Vigneron, M., Kedinger, C. Chromosomal localization of human RNA polymerase II subunit genes. Genomics 20: 496-499, 1994. [PubMed: 8034326] [Full Text: https://doi.org/10.1006/geno.1994.1208]

  2. Acker, J., Wintzerith, M., Vigneron, M., Kedinger, C. Primary structure of the second largest subunit of human RNA polymerase II (or B). J. Molec. Biol. 226: 1295-1299, 1992. [PubMed: 1518060] [Full Text: https://doi.org/10.1016/0022-2836(92)91071-v]

  3. Hsin, J.-P., Sheth, A., Manley, J. L. RNAP II CTD phosphorylated on threonine-4 is required for histone mRNA 3-prime end processing. Science 334: 683-686, 2011. [PubMed: 22053051] [Full Text: https://doi.org/10.1126/science.1206034]


Creation Date:
Victor A. McKusick : 6/23/1993

Edit History:
alopez : 11/30/2011
terry : 11/29/2011
carol : 6/19/2001
alopez : 6/19/2001
carol : 6/19/2001
alopez : 6/19/2001
alopez : 6/19/2001
carol : 6/15/2001
psherman : 9/2/1999
carol : 6/22/1998
joanna : 4/4/1996
carol : 4/18/1994
carol : 6/23/1993



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OMIM® and Online Mendelian Inheritance in Man® are registered trademarks of the Johns Hopkins University.
Copyright® 1966-2024 Johns Hopkins University.

NOTE: OMIM is intended for use primarily by physicians and other professionals concerned with genetic disorders, by genetics researchers, and by advanced students in science and medicine. While the OMIM database is open to the public, users seeking information about a personal medical or genetic condition are urged to consult with a qualified physician for diagnosis and for answers to personal questions.
OMIM® and Online Mendelian Inheritance in Man® are registered trademarks of the Johns Hopkins University.
Copyright® 1966-2024 Johns Hopkins University.
Printed: May 4, 2024