Alternative titles; symbols
HGNC Approved Gene Symbol: POLR2C
Cytogenetic location: 16q21 Genomic coordinates (GRCh38): 16:57,462,679-57,472,009 (from NCBI)
The eukaryotic DNA-dependent RNA polymerase II (or B) is composed of 10 to 14 polypeptides ranging from 220 to 10 kD. For general information on the structure and function of RNA polymerase II, see 180660.
Pati and Weissman (1990) cloned a cDNA encoding the 33-kD subunit of human RNA polymerase II (POLR2C) by screening a human placenta cDNA library with oligonucleotides based on the peptide sequence of the bovine 33-kD subunit. The deduced 275-amino acid human POLR2C protein, termed RPB33 by the authors, shares 45% sequence identity with the yeast homolog RPB3. POLR2C contains a single cysteine-rich region. Northern blot analysis of HeLa cell mRNA detected an approximately 1.8-kb POLR2C doublet.
Acker et al. (1994) isolated cDNAs for 5 human RNA polymerase II subunits, including the 33-kD subunit.
Dammann and Pfeifer (1998) cloned a human POLR2C cDNA. They reported that the POLR2C protein shares 40% sequence homology with the 40-kD subunit of human RNA polymerase I.
Using the yeast 2-hybrid system with the 13.3-kD subunit of human RNA polymerase II (POLR2J; 604150) as bait, Fanciulli et al. (1998) isolated human skeletal muscle cDNAs corresponding to 2 distinct POLR2C mRNAs which differ in the length of their 3-prime untranslated regions. The authors demonstrated that POLR2C and POLR2J can interact in mammalian cells. By Northern blot analysis, they found that POLR2C is ubiquitously expressed in human tissues, with the highest expression in skeletal muscle, heart, and pancreas. POLR2C mRNA expression was sensitive to the antineoplastic antibiotic doxorubicin.
By in situ hybridization, Acker et al. (1994) mapped the POLR2C gene to 16q13-q21.
Acker, J., Mattei, M.-G., Wintzerith, M., Roeckel, N., Depetris, D., Vigneron, M., Kedinger, C. Chromosomal localization of human RNA polymerase II subunit genes. Genomics 20: 496-499, 1994. [PubMed: 8034326] [Full Text: https://doi.org/10.1006/geno.1994.1208]
Dammann, R., Pfeifer, G. P. Cloning and characterization of the human RNA polymerase I subunit hRPA40. Biochim. Biophys. Acta 1396: 153-157, 1998. [PubMed: 9540830] [Full Text: https://doi.org/10.1016/s0167-4781(97)00206-6]
Fanciulli, M., Bruno, T., Di Padova, M., De Angelis, R., Lovari, S., Floridi, A., Passananti, C. The interacting RNA polymerase II subunits, hRPB11 and hRPB3, are coordinately expressed in adult human tissues and down-regulated by doxorubicin. FEBS Lett. 427: 236-240, 1998. [PubMed: 9607318] [Full Text: https://doi.org/10.1016/s0014-5793(98)00431-1]
Pati, U. K., Weissman, S. M. The amino acid sequence of the human RNA polymerase II 33-kDa subunit hRPB 33 is highly conserved among eukaryotes. J. Biol. Chem. 265: 8400-8403, 1990. [PubMed: 2187864]