HGNC Approved Gene Symbol: SURF2
Cytogenetic location: 9q34.2 Genomic coordinates (GRCh38): 9:133,356,550-133,361,158 (from NCBI)
Using mouse Surf2 cDNA to screen placenta and HeLa cell cDNA libraries, followed by database analysis and 3-prime RACE of a HeLa cDNA library, Lennard et al. (1994) cloned human SURF2. The deduced 256-amino acid protein shares 69% identity with mouse Surf2.
Lennard et al. (1994) found that the major start sites of the SURF1 (185620) and SURF2 genes are separated by 97 bp, although both genes have multiple transcription start sites. The intergenic region is expected to have bidirectional promoter activity, as is found in mouse. This region lacks a TATA box, but is GC-rich. DNase footprint analysis showed 4 regions that interacted with HeLa cell nuclear factor complexes.
Duhig et al. (1998) stated that the 5-prime end of each of the surfeit genes, including SURF2, is contained within a CpG island. Although the mouse Surf2 and Surf4 (185660) genes overlap at their 3-prime ends, human SURF2 and SURF4 are separated by 302 bp due to a much shorter 3-prime UTR in the human SURF2 gene.
Using FISH, Yon et al. (1993) mapped the SURF2 gene within the surfeit gene cluster on chromosome 9q34. They stated that the mouse surfeit gene cluster maps to the proximal portion of chromosome 2.
Duhig et al. (1998) determined that the human surfeit gene cluster spans 60 kb on chromosome 9q34.
Duhig, T., Ruhrberg, C., Mor, O., Fried, M. The human surfeit locus. Genomics 52: 72-78, 1998. [PubMed: 9740673] [Full Text: https://doi.org/10.1006/geno.1998.5372]
Lennard, A., Gaston, K., Fried, M. The Surf-1 and Surf-2 genes and their essential bidirectional promoter elements are conserved between mouse and human. DNA Cell Biol. 13: 1117-1126, 1994. [PubMed: 7702754] [Full Text: https://doi.org/10.1089/dna.1994.13.1117]
Yon, J., Jones, T., Garson, K., Sheer, D., Fried, M. The organization and conservation of the human Surfeit gene cluster and its localization telomeric to the c-abl and can proto-oncogenes at chromosome band 9q34.1. Hum. Molec. Genet. 2: 237-240, 1993. [PubMed: 8499913] [Full Text: https://doi.org/10.1093/hmg/2.3.237]