Alternative titles; symbols
HGNC Approved Gene Symbol: GCFC2
Cytogenetic location: 2p12 Genomic coordinates (GRCh38): 2:75,662,705-75,713,122 (from NCBI)
Kageyama and Pastan (1989) cloned a cDNA for a human transcriptional regulator, which they called GCF, that represses transcription of certain genes and is encoded by a 3-kb mRNA.
Johnson et al. (1992) found that the GCF gene is widely expressed in human tissues and cell lines.
Takimoto et al. (1999) also cloned a GCF cDNA and found 3 major revisions in the sequence originally published by Kageyama and Pastan (1989). By 5-prime RACE, Takimoto et al. (1999) determined that the 5-prime end is 308 nucleotides shorter than the original sequence, that a 'T' at position 787 is not observed, and that there is an insertion of 114 nucleotides between positions 851 and 852 of the previous sequence. The revisions resulted in a cDNA consisting of 2,661 nucleotides encoding a 781-amino acid protein. SDS-PAGE analysis determined that GCF is expressed as an approximately 120-kD protein. Gel shift analysis also revealed that the revised GCF has no sequence-specific DNA-binding activity. Takimoto et al. (1999) concluded that to avoid errors in the sequencing it is important to confirm cDNA sequences by isolating and sequencing more than 1 clone and by comparing the cDNA with the genomic DNA sequence.
By in situ hybridization, Johnson et al. (1992) mapped the GCF gene to chromosome 2p11.2-p11.1.
Johnson, A. C., Kageyama, R., Popescu, N. C., Pastan, I. Expression and chromosomal localization of the gene for the human transcriptional repressor GCF. J. Biol. Chem. 267: 1689-1694, 1992. [PubMed: 1370479]
Kageyama, R., Pastan, I. Molecular cloning and characterization of a human DNA binding factor that represses transcription. Cell 59: 815-825, 1989. [PubMed: 2556218] [Full Text: https://doi.org/10.1016/0092-8674(89)90605-3]
Takimoto, M., Mao, P., Wei, G., Yamazaki, H., Miura, T., Johnson, A. C., Kuzumaki, N. Molecular analysis of the GCF gene identifies revisions to the cDNA and amino acid sequences. Biochim. Biophys. Acta 1447: 125-131, 1999. [PubMed: 10500253] [Full Text: https://doi.org/10.1016/s0167-4781(99)00127-x]