Alternative titles; symbols
HGNC Approved Gene Symbol: NFYB
Cytogenetic location: 12q23.3 Genomic coordinates (GRCh38): 12:104,117,086-104,138,210 (from NCBI)
NFYB is a subunit of a conserved transcription factor that binds specifically to CCAAT elements in promoters (Chen et al., 2011).
Chen et al. (2011) had found that expression of DNA topoisomerase-2 (TOP2)-alpha (TOP2A; 126430) was inverse to expression of NFYB. They found reduced TOP2-alpha and elevated NFYB expression in a CEM human lymphoblastic leukemia subline that was resistant to teniposide, a TOP2-alpha-targeting chemotherapeutic agent. MicroRNA profiling revealed reduced expression of MIR485-3p (615385) in teniposide-resistant cells. Overexpression of MIR485-3p downregulated NFYB via an MIR485-3p-binding site in the 3-prime UTR of the NFYB transcript, resulting in upregulated TOP2-alpha expression and restored sensitivity to teniposide. Overexpression of MIR485-3p also enhanced etoposide sensitivity in etoposide-resistant human rhabdomyosarcoma cells and in teniposide-resistant CEM cells. Chen et al. (2011) concluded that MIR485-3p-dependent downregulation of NFYB enhances cell sensitivity to TOP2-alpha inhibitors.
Li et al. (1991) assigned the NFYB gene to human chromosome 12 by in situ hybridization. They mapped the mouse gene to chromosome 10.
Gross (2013) mapped the NFYB gene to chromosome 12q23.3 based on an alignment of the NFYB sequence (GenBank BC005316) with the genomic sequence (GRCh37).
Chen, C.-F., He, X., Arslan, A. D., Mo, Y.-Y., Reinhold, W. C., Pommier, Y., Beck, W. T. Novel regulation of nuclear factor-YB by miR-485-3p affects the expression of DNA topoisomerase II-alpha and drug responsiveness. Molec. Pharm. 79: 735-741, 2011. [PubMed: 21252292] [Full Text: https://doi.org/10.1124/mol.110.069633]
Gross, M. B. Personal Communication. Baltimore, Md. 8/28/2013.
Li, X.-Y., Mattei, M. G., Zaleska-Rutczynska, Z., Hooft van Huijsduijnen, R., Figueroa, F., Nadeau, J., Benoist, C., Mathis, D. One subunit of the transcription factor NF-Y maps close to the major histocompatibility complex in murine and human chromosomes. Genomics 11: 630-634, 1991. [PubMed: 1774067] [Full Text: https://doi.org/10.1016/0888-7543(91)90070-u]