Alternative titles; symbols
HGNC Approved Gene Symbol: POLR2G
Cytogenetic location: 11q12.3 Genomic coordinates (GRCh38): 11:62,761,580-62,766,710 (from NCBI)
RNA polymerase II is the enzymatic complex responsible for transcription of genes that result in mRNA production. For general information on the structure and function of RNA polymerase II, see 180660.
Schoen et al. (1997) stated that 7 of the RNA polymerase II complex genes had been isolated and assigned to chromosomal sites. The complete genomic sequence had been reported for only the 220-kD (POLR2A; 180660) and 14.5-kD (POLR2I; 180662) subunits. Schoen et al. (1997) cloned, sequenced, and mapped the seventh subunit of the RNA polymerase II complex, which they designated hsRPB7. Southern blots of genomic and cloned DNA suggested that it is encoded by a single gene. The sequence of the 5-prime flanking region did not contain a TATA element, but did contain several Sp1-binding sites, an AP-1 site, and a novel inverted polymorphic GATA tandem repeat. Schoen et al. (1997) stated that this novel GATA repeat should be useful for linkage analysis. The gene seemed to be highly conserved among eukaryotic species, showing general sequence conservation with yeast and Drosophila. Northern blot analysis revealed a high degree of tissue-specific expression. Expression was relatively high in adult retina, brain, and kidney.
The POLR2G gene consists of 8 exons and spans approximately 5.1 kb (Schoen et al., 1997).
Khazak et al. (1998) demonstrated that POLR2D (606017) associates strongly and specifically with POLR2G in the nuclei of human cells. The association appeared to require sequences spanning the N-terminal half of the POLR2D protein, whereas no truncation of POLR2G was observed to associate with POLR2D.
Using human radiation hybrids and YACs, Schoen et al. (1997) localized the POLR2G gene to 11q13.1, within 70 kb of marker D11S1765.
Khazak, V., Estojak, J., Cho, H., Majors, J., Sonoda, G., Testa, J. R., Golemis, E. A. Analysis of the interaction of the novel RNA polymerase II (pol II) subunit hsRPB4 with its partner hsRPB7 and with pol II. Molec. Cell. Biol. 18: 1935-1945, 1998. [PubMed: 9528765] [Full Text: https://doi.org/10.1128/MCB.18.4.1935]
Schoen, T. J., Chandrasekharappa, S. C., Guru, S. C., Mazuruk, K., Chader, G. J., Rodriguez, I. R. Human gene for the RNA polymerase II seventh subunit (hsRPB7): structure, expression and chromosomal localization. Biochim. Biophys. Acta 1353: 39-49, 1997. [PubMed: 9256063] [Full Text: https://doi.org/10.1016/s0167-4781(97)00041-9]