Gene: [01p32/RLF] rearranged L-myc fusion protein;
COM |
The sequence, included in this entry, corresponds only partly to the gene/protein in question since so far complete cDNA of the gene has not been yet published." |
SUM |
This protein is related to members of the MYC family. Initially, it was revealed as a part of chimeric polypeptides with amino-terminal sequences derived from a novel gene, named RLF (Rearranged Lmyc Fusion) since its protein has been found to be linked with the Lmyc protein only in cells with an amplified LMYC gene (Makela-1991). Also, since the chimeric products of the RLF/MYCL fusion gene were found in different small cell lung carcinoma (SCLC) cell lines, the authors suggested a role for the fusion protein in oncogenesis. The authors cloned a cDNA encoding the MYCLRF protein itself, and also established a physical linkage between this gene and L-myc-1 on Chr 1 within the band p32. The both genes appeared to be separated by less than 800 kb." |
COD |
"<* TITLE:> [PROT= 96? aa (MW= ?) from cDNA= 300 bp] <* REFER_1:> [Makela TP &:1991] /IDP: Makela TP, Saksela K, Evan G, Alitalo K; 'A fusion protein formed by L-myc and a novel gene in SCLC'; EMBO J, 10, N6, 1331-1335, 1991\ /IDS_1: emb:X58480^HSRLF| cDNA= 300 bp; 'Human rlf mRNA for l-myc associated fusion protein'; //note: seq_from cell_line GLC28\ /Date: May-15-1991\ /Submitted_by: Maekelae TP (Cancer Biology Lab, Depts Virology and Pathology, University of Helsinki, Haartmaninkatu 3, 00290 Helsinki, Finland); //date: Mar-22-1991; //note: only 1..249 nt\ /Duplicate: cbi:36069-070\ /Note_1: The last name 'Maekelae' indicated in this emb:entry should probably be 'Makela' as in the original publication.\ /Note_2: The following comment is selected from this emb:entry - 'A breakpoint is found at position 249 bp, and corresponds to nu- cleotide 796 L-myc of Kaye F, Battey J, Nau M, Brooks B, Seifter E, De Greve J, Birrer M, Sausville E and Minna J. Mol Cell Biol, 8, 186-195 (1988).' However, without additional clarifications, this comment may orig- inates a confusion (see '/Note:' in 'Sequence' below).\ %----------------------------------------------------------------------- <* LANDMARKS> {seg(<1..12) == 5'-UTL(=12) //: 5'-UnTransLated region (possibly incomplete); seg(13..300>) == CDS(=288) //: CoDing_Segment (for 96 aa only); pos:13 == TLI_1 //: pos:246|247 == BFP_1 //: Break-Fusion_Point} %----------------------------------------------------------------------- <* SEQUENCE:> [cDNA= 300 bp] /Note: The most probable site of the mentioned 'break-fusion_point' is restricted by nucleotides #246|247, in the ref1 sequence, and by #792|793, in the ref2 sequence, where ref2 is (Kaye F &:1988). Also, in the ref2 sequence, seg(784..795) is a terminal segment of the intron A (or 1) while seg(796..804) is a 5'-untranslated part of the exon 2 (see 'Gene_structure' in GEM:01p32/MYCL1). Thus, the L-myc-1's part begins at the position #83 in the fusion protein, or at the position #259 in the nucleotide sequence below.\ %------- ids1: 1=cgccgtggga ag=12_ %------- pep1: 1= M A D G K G D A A A V A G A G = ids1: 13=atg gca gac gga aag gga gac gcc gcc gct gtc gcc ggg gct ggg= pep1: 16= A E A P A V A G A G D G V E T = ids1: 58=gct gag gct ccg gcg gta gcg gga gcc gga gat gga gtc gag act= pep1: 31= E S M V R G H R P V S P A P G = ids1:103=gag tcc atg gtt cgg ggt cat cgc ccc gta tct cca gcg ccg gga= pep1: 46= A S G L R P C L W Q L E T E L = ids1:148=gcc tcg gga ctg cgg ccg tgt ctg tgg cag ctg gag aca gag ctg= pep1: 61= R E Q E V S E V S S L N Y C R = ids1:193=agg gag caa gag gtg tcg gag gtc tca tct ttg aac tac tgc cgg= pep1: 76= S F C =78_ ids1:238=AGc Ttc TGC=246_ #ref2:784=TCc Ctc CCG=792_ %------- pep1: 79= Q G A D M D Y D S Y Q H Y F Y = ids1:247=cag gga gcg gac atg gac tac gac tcg tac cag cac tat ttc tac= #ref2:793=cag gga gcg gac atg gac tac gac tcg tac cag cac tat ttc tac= #pep2: 1=_________________M D Y D S Y Q H Y F Y = //note: L-myc-1 protein ^ is as follows ..\ %------- pep1: 94= D Y D =96\ ids1:292=gac tat gac=300\ #pep2: 12= D Y D C =15..| #ref2:838=gac tat gac tgc=849..|" |
PSG |
GEM:00.0/RLFP. |
REL |
GEM:01p32/MYCL1. |
REF |
COM,SQN "Kaye F &: Mol Cell Biol, 8, 186-195, 1988 CLO,COD,SEQ,LOC,MOL "Makela TP &: Mol Cell Biol, 11, 4015-4021, 1991 |
KEY |
onc, fus |
CLA |
coding, basic |
LOC |
01 p32 |
MIM |
MIM: 180610 |