Gene: [01p/ARH9] protooncogene ARH-9 (RHOC; Aplysia ras-related homolog 9); [RHOH9 rhoC ]

COM

ARH9 (originally rhoC), a member of the RHO gene subfamily (in turn, belonging to the RAS superfamily), was initially assigned to the telomeric region of Chr 5q by isotopic chromosomal in situ hybridization and Southern analysis of somatic cell hybrid DNAs (Cannnizzaro-1990; Huebner-1990). In those experiments, the gene was noted to cosegregate 100% concordantly with the human CSF1 gene in somatic cell hybrids carrying partial Chr 5, together with other human chromosomes. Since the reassignment of the CSF1 locus from Chr 5q to Chr 1p has recently been performed (Morris-1991; see GEM:01p/CSF1), the same authors also reexamined the localization of the ARH9 gene. New results demonstrated that ARH9 was present in hybrids retaining Chr 1p, but not 5 (Morris-1993). The authors mapped ARH9 relative to other 1p loci, localizing the gene to the region 1p13-p31. Fluorescence in situ hybridization (FISH) to metaphase chromosomes with a 12 kb genomic ARH9 clone confirmed the gene's localization to Chr 1, within bands p21-p13. Explaining the previous erroneous localization of the gene (based on usage of isotopic in situ hybridization), the authors suggested that this error 'emphasizes a significant limitation of this technique, particularly, the low signal-to-noise ratio' in comparing with the FISH method."

PRT

"[1] Sequence: 193 aa; 22,006 Da.
------------------------------------------------------------------
From: Chardin P, Madaule P, Tavitian A;
Coding nucleotide of human rho cDNAs clone 6 and clone 9;
Nucleic Acids Research, 16, 2717-2717, 1988.
---------------........----------------_________---------
{MAAIRKKLVIVCLLIVFSKDQFPEVIADIEVDG = 50
KQVELALWEDYDRLRPLSYPDTDVI LMCFSIDSPD SLENIPEKWT =100
PEVKHFPIILVGNKKDLRQDEHTRRE LAKMKQEPVR SEEGRDMANR =150
ISAFGYLECS AKTKEGVREV FEMATRAGLQ VRKNKRRRGPIL} =193
---------------------------------------------------------
Features:
seg(12..19) == NP-binding (GTP)site;
seg(34..42) == Effector domain (putative);
seg(59..63) == NP-binding (GTP)site;
seg(117..120) == NP-binding (GTP)site;
pos(190) == Geranyl-Geranyl lipid-binding site.
------------------------------------------------------------------
[2] Cross-References (Protein DBs):
swp:P08134/RHOC_HUMAN/Aug-01-1988/Oct-01-1993;
{transforming protein RHOC (H9); ARH9}.
pir:S01029; pir:TVHURC."

GEN

[1] cDNA 582 bp {gnb:X06821/HSRHOC9/Feb-15-1990; human rhoC mRNA (clone 9); GTP-binding protein; ras-related protein; rhoC gene}. ------------------------------------------------------------------ From: Chardin P, Madaule P, Tavitian A; Coding nucleotide of human rho cDNAs clone 6 and clone 9; Nucleic Acids Research, 16, 2717-2717, 1988. ------------------------------------------------------------------ Features: start: {1=atg}; CDS(=579): {1..579 nt --> 1..193 aa}; stop: {580=tga}. ------------------------------------------------------------------
[2] Cross-References (DNA DBs): emb:X06821/HSRHOC9/Jul-16-1988/Sep-12-1993; {human rhoC mRNA (clone 9)}; submitted (Feb-12-1988) to EMBL/GenBank/DDBJ by Chardin P, INSERM U-248, Faculte de Medecine, Lariboisiere Saint-Louis, 10 av. de Verdun 75010 Paris, France."

REL

GEM:03p213/ARHA; GEM:02p/ARHB.

REF

LOC,EAG "Cannizzaro LA &: Genomics, 6, N2, 197-203, 1990
COD,SEQ "Chardin P &: NAR, 16, 2717, 1988
LOC,FAG "Huebner K &: AJHG, 46, N1, 26-36, 1990
COD,SEQ,EVO "Madaule P, Axel R: Cell, 41, 37-41, 1985
LOC,FAG "Morris SW &: Genomics, 15, N3, 677-679, 1993

SWI

SWISSPROT: P08134

KEY

onc

CLA

coding, basic

LOC

01 p21-13

MIM

MIM: 165380

SYN

RHOH9 rhoC